Unità di Ricerca: Fisiologia (Alloatti-Pagliaro-Roatta)
Coordinatore: Prof. Claudia Penna
Delegato del Rettore: Prof Pasquale Pagliaro
Sede Universitaria: Università di Torino, Dipartimento di Scienze della Vita e Biologia dei Sistemi
Dipartimento di Scienze Cliniche e Biologiche e Dipartimento di Neuroscienze.
Indirizzo : Via Accademia Albertina 13
10123 Torino Italy
tel: 0116704666
fax: 0116704508
e-mail: giuseppe.alloatti@unito.it
sito web: http://www.dbios.unito.it/do/docenti.pl/Show?_id=galloatt#profilo
Linee di ricerca:
Meccanismi e vie intracellulari di cardio-protezione. Meccanismo d’azione di mediatori peptidici derivati dalla Cromogranina A con effetti cardioprotettivi. Biomateriali e cardiorigenerazione.
Meccanismi di controllo della perfusione muscolare e vaso-reattività alla stimolazione pneumatica.
Laboratori/strumentazione:
| LABORATORI | STRUMENTAZIONE |
| Labs fisiologia cellulare e di fisiologia cardiaca | Sistema di perfusione per cuori isolati (ratti e topi)
Attrezzatura per l’isolamento di cellule cardiache e cellule staminali mesenchimali in condizioni di sterilità Mantenimento in coltura di cellule cardiache e cellule staminali mesenchimali. Attrezzatura per l’isolamento di mitocondri da cellule cardiache e cellule staminali Citofluorimetro CAIRN montato su microscopio rovesciato Zeiss e amplificatore da patch-clamp per la misura contemporanea di correnti di membrana e del calcio intracellulare Camera Ipossica per colture cellulari e piccoli animali Microscopio confocale e confocale invertito (Olympus fluoview) Microscopio elettronico ChemiDoc Touch |
| Lab Fisiologia Integrativa | Amplificatore optoisolato 8 ch (EEG, ECG, EMG,…)
Sistema di acquisizione 16 ch CED 1401micro Pedana stabilometrica Near infrared spectroscopy (in prestito) Flussimetro cutaneo laser-Doppler Ecografo ESAOTE Mylab 25Gold Sistema custom di compressione pneumatica degli arti |
Collaborazioni internazionali:
Claudia Montero-Menei, University of Angers, Francia
Sushil K. Mahata, University of California; USA
Nazareno Paolocci Johns Hopkins University, Baltimore, USA
Elenco pubblicazioni del triennio 2016 – 2018.
1) Femminò, S, Penna, C, Bessone, F, Caldera, F, Dhakar, N, Cau, D, Pagliaro, P, Cavalli, R, Trotta, F. α-cyclodextrin and α-cyclodextrin polymers as oxygen nanocarriers to limit hypoxia/reoxygenation injury: Implications from an in vitro model. Polymers Volume 10, Issue 2, 22 February 2018, Article number 211 (Open Access).
2) Angotti C, Venier-Julienne MC, Penna C, Femminò S, Sindji L, Paniagua C, Montero-Menei CN, Pagliaro P. Nanoprecipitated catestatin released from pharmacologically active microcarriers (PAMs) exerts pro-survival effects on MSC. Int J Pharm. 2017 May 25;523(2):506-514. doi: 10.1016/j.ijpharm.2016.11.050.
3) Cadeddu C, Mercurio V, Spallarossa P, Nodari S, Triggiani M, Monte I, Piras R, Madonna R, Pagliaro P, Tocchetti CG, Mercuro G. Preventing antiblastic drug-related cardiomyopathy: old and new therapeutic strategies. J Cardiovasc Med (Hagerstown). 2016 May;17 Suppl 1:S64-75. doi: 10.2459/JCM.0000000000000382.
4) Deidda M, Madonna R, Mango R, Pagliaro P, Bassareo PP, Cugusi L, Romano S, Penco M, Romeo F, Mercuro G. Novel insights in pathophysiology of antiblastic drugs-induced cardiotoxicity and cardioprotection. J Cardiovasc Med (Hagerstown). 2016 May;17 Suppl 1:S76-83. doi: 10.2459/JCM.0000000000000373.
5) Folino A, Accomasso L, Giachino C, Montarolo PG, Losano G, Pagliaro P, Rastaldo R. Apelin-induced cardioprotection against ischaemia/reperfusion injury: roles of epidermal growth factor and Src. Acta Physiol (Oxf). 2018 Feb;222(2). doi: 10.1111/apha.12924.
6) Folino A, Benzo M, Pasquero P, Laguzzi A, Mesin L, Messere A, Porta M, Roatta S. Vena cava responsiveness to controlled isovolumetric respiratory efforts, J Ultras Med. 36:2113-2123, 201
7) Gesmundo I, Miragoli M, Carullo P, Trovato L, Larcher V, Di Pasquale E, Brancaccio M, Mazzola M, Villanova T, Sorge M, Taliano M, Gallo Mp, Alloatti G, Penna C, Hare Jm, Ghigo E, Schally Av, Condorelli G, Granata R. Growth hormone-releasing hormone attenuates cardiac hypertrophy and improves heart function in pressure overload heart failure. PNAS 114(45): 12033-12038, 2017 doi: 10.1073/pnas.1712612114.
8) Mastrocola R, Aragno M, Alloatti G, Collino M, Penna C, Pagliaro P. Metaflammation: tissue-specific alterations of the NLRP3 inflammasome platform in metabolic syndrome. Curr Med Chem. 2017 Apr 7. doi: 10.2174/0929867324666170407123522.
9) Mastrocola R, Collino M, Penna C, Nigro D, Chiazza F, Fracasso V, Tullio F, Alloatti G, Pagliaro P, Aragno M. Maladaptive Modulations of NLRP3 Inflammasome and Cardioprotective Pathways Are Involved in Diet-Induced Exacerbation of Myocardial Ischemia/Reperfusion Injury in Mice. Oxid Med Cell Longev. 2016;2016:3480637. doi: 10.1155/2016/3480637. Epub 2015 Dec 14.
10) Mastrocola R, Penna C, Tullio F, Femmino’ S, Nigro D, Chiazza F, Serpe L, Alloatti G, Cocco M, Bertinaria M, Pagliaro P, Aragno M, Collino M. Pharmacological inhibition of NLRP3 inflammasome attenuates myocardial ischemia/reperfusion injury by activation of RISK and mitochondrial pathways. Oxidative Medicine and Cellular Longevity, special issue “Oxidative Stress and Inflammation in Cardiovascular Disease”, volume 2016, Article ID 5271251, 11 pages, 2016. doi:10.1155/2016/5271251
11) Maurea N, Spallarossa P, Cadeddu C, Madonna R, Mele D, Monte I, Novo G, Pagliaro P, Pepe A, Tocchetti CG, Zito C, Mercuro G. A recommended practical approach to the management of target therapy and angiogenesis inhibitors cardiotoxicity: an opinion paper of the working group on drug cardiotoxicity and cardioprotection, Italian Society of Cardiology. J Cardiovasc Med (Hagerstown). 2016 May;17 Suppl 1 Special issue on Cardiotoxicity from Antiblastic Drugs and Cardioprotection:e93-e104.
12) Mele D, Tocchetti CG, Pagliaro P, Madonna R, Novo G, Pepe A, Zito C, Maurea N, Spallarossa P. Pathophysiology of anthracycline cardiotoxicity. J Cardiovasc Med (Hagerstown). 2016 May;17 Suppl 1:S3-S11. doi: 10.2459/JCM.0000000000000378.
13) Messere A, Ceravolo G, Franco W, Maffiodo D, Ferraresi C, Roatta S. Increased tissue oxygenation explains the attenuation of hyperaemia upon repetitive pneumatic compression of the lower leg. J Appl Physiol (1985). 123(6):1451-1460, 2017. doi: 10.1152/japplphysiol.00511.201
14) Messere A, Turturici M, Millo G, Roatta S. Repetitive muscle compression reduces vascular mechano-sensitivity and the hyperemic response to muscle contraction. J Physiol Pharmacol. 68:427-437, 2017
15) Morano M, Angotti C, Tullio F, Gambarotta G, Penna C, Pagliaro P, Geuna S. Myocardial ischemia/reperfusion upregulates the transcription of the Neuregulin1 receptor ErbB3, but only postconditioning preserves protein translation: Role in oxidative stress. Int J Cardiol. 2017 Apr 15;233:73-79. doi: 10.1016/j.ijcard.2017.01.122.
16) Novo G, Cadeddu C, Sucato V, Pagliaro P, Romano S, Tocchetti CG, Zito C, Longobardo L, Nodari S, Penco M. Role of biomarkers in monitoring antiblastic cardiotoxicity. J Cardiovasc Med (Hagerstown). 2016 May;17 Suppl 1 Special issue on Cardiotoxicity from Antiblastic Drugs and Cardioprotection:e27-e34.
17) Pagliaro P, Penna C. Hypertension, hypertrophy, and reperfusion injury. J Cardiovasc Med (Hagerstown). 2017 Mar;18(3):131-135. doi: 10.2459/JCM.0000000000000435.
18) Penna C, Tullio F, Femminò S, Rocca C, Angelone T, Cerra Mc, Gallo MP, Gesmundo I, Fanciulli A, Brizzi MF, Pagliaro P, Alloatti G, Granata R. Obestatin regulates cardiovascular function and promotes cardioprotection through the nitric oxide pathway. J Cell Mol Med. 21: 3670-3678, 2017. doi: 10.1111/jcmm.13277.
19) Pergolizzi B, Carriero V, Abbadessa G, Penna C, Berchialla P, De Francia S, Bracco E, Racca S. Subchronic nandrolone administration reduces cardiac oxidative markers during restraint stress by modulating protein expression patterns. Mol Cell Biochem. 2017 Oct;434(1-2):51-60. doi: 10.1007/s11010-017-3036-7.
20) Quintieri AM, Filice E, Amelio D, Pasqua T, Lupi FR, Scavello F, Cantafio P, Rocca C, Lauria A, Penna C, De Cindio B, Cerra MC, Angelone T. The innovative “Bio-Oil Spread” prevents metabolic disorders and mediates preconditioning-like cardioprotection in rats. Nutr Metab Cardiovasc Dis. 2016 Jul;26(7):603-613. doi:10.1016/j.numecd.2016.02.009.
21) Russo I, Penna C, Musso T, Popara J, Alloatti G, Cavalot F, Pagliaro P. Platelets, diabetes and myocardial ischemia/reperfusion injury. Cardiovasc Diabetol. 2017 May 31;16(1):71. doi: 10.1186/s12933-017-0550-6.
22) Spallarossa P, Maurea N, Cadeddu C, Madonna R, Mele D, Monte I, Novo G, Pagliaro P, Pepe A, Tocchetti CG, Zito C, Mercuro G. A recommended practical approach to the management of anthracycline-based chemotherapy cardiotoxicity: an opinion paper of the working group on drug cardiotoxicity and cardioprotection, Italian Society of Cardiology. J Cardiovasc Med (Hagerstown). 2016 May;17 Suppl 1 Special issue on Cardiotoxicity from Antiblastic Drugs and Cardioprotection:e84-e92.
23) Testa M, Di Marco A, Pertusio R, Van Roy P, Cattrysse E, Roatta S. A validation study of a new instrument for low cost bite force measurement. J Electromyogr Kinesiol. 30:243-8, 2016
24) Tocchetti CG, Cadeddu C, Di Lisi D, Femminò S, Madonna R, Mele D, Monte I, Novo G, Penna C, Pepe A, Spallarossa P, Varricchi G, Zito C, Pagliaro P, Mercuro G. From Molecular Mechanisms to Clinical Management of Antineoplastic Drug-Induced Cardiovascular Toxicity: A Translational Overview. Antioxid Redox Signal. 2017 May 15. doi: 10.1089/ars.2016.6930.
25) Tullio F, Penna C, Cabiale K, Femminò S, Galloni M, Pagliaro P. Cardioprotective effects of calcitonin gene-related peptide in isolated rat heart and in H9c2 cells via redox signaling. Biomed Pharmacother. 2017 Jun;90:194-202. doi: 10.1016/j.biopha.2017.03.043.